Mycobacterium tuberculosis Nucleic Acid iyo Rifampicin (RIF) , iska caabinta (INH)

Sharaxaad Gaaban:

Qalabkan waxaa loo isticmaalaa in vitro lagu ogaado tayada Mycobacterium DNA ee xaakada bini'aadamka, dhaqanka adag (LJ Dhexdhexaadiyaha) iyo dhaqanka dareeraha ah (MGIT Medium), dheecaanka boronkiitada, iyo isbeddellada ku jira 507-533 amino acid codeon (81bp, rifampicin go'aaminta caabbinta gobolka ee MycopoB). Isbeddellada ku jira goobaha ugu muhiimsan ee qaaxada Mycobacterium isoniazid resistance.Waxay gargaar siisaa ogaanshaha infekshanka qaaxada Mycobacterium, waxayna ogaataa hidda-wadaha iska caabinta ugu weyn ee rifampicin iyo isoniazid, taas oo gacan ka geysaneysa fahamka caabbinta daawada ee qaaxada Mycobacterium ee uu buka buka.

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HWTS-RT147 Mycobacterium Tuberculosis Nucleic Acid iyo Rifampicin(RIF), (INH) Qalabka lagu ogaado (qallooca dhalaalay)

Epidemiology

Mycobacterium tuberculosis, oo loo yaqaan tubercle bacillus (TB), waa bakteeriyada keenta cudurka qaaxada, hadda, daawaynta safka hore ee ka hortagga qaaxada waxaa ka mid ah isoniazid, rifampicin iyo ethambutol, iwm.^[1]. Si kastaba ha ahaatee, iyadoo ay ugu wacan tahay isticmaalka khaldan ee dawooyinka ka hortagga qaaxada iyo sifooyinka qaab dhismeedka gidaarka unugga ee qaaxada mycobacterium laftiisa, qaaxada mycobacterium waxay soo saartay caabbinta daawooyinka ka hortagga qaaxada, iyo qaabka gaarka ah ee khatarta ah waa qaaxada u adkeysata daawooyinka badan (MDR-TB), taas oo u adkaysata labada dawo ee ugu caansan uguna waxtarka badan, rimpidcin^[2].

Dhibaatada iska caabbinta daawada qaaxada waxay ka jirtaa dhammaan wadamada ay WHO sahan ku samaysay. Si loo helo qorshayaal daaweyn oo sax ah oo loogu talagalay bukaannada qaaxada, waxaa lagama maarmaan ah in la ogaado caabbinta daawooyinka ka hortagga qaaxada, gaar ahaan iska caabbinta rifampicin, taas oo noqotay tallaabo lagu ogaanayo oo ay WHO ku talisay daaweynta qaaxada.^[3]. Inkasta oo helitaanka iska caabbinta rifampicin ay ku dhowdahay helitaanka MDR-TB, kaliya ogaanshaha iska caabbinta rifampicin ayaa iska indha tiraya bukaannada qaba mono-u adkaysta INH (oo loola jeedo iska caabbinta isoniazid laakiin u nugul rifampicin) iyo rifampicin mono-adkeysiga (dareenka isoniazid laakiin iska caabinta rifampicin), taas oo laga yaabo inay u horseedi karto bukaannada daaweynta aan la hagaajin karin. Sidaa darteed, tijaabooyinka iska caabbinta isoniazid iyo rifampicin ayaa ah shuruudaha ugu yar ee lagama maarmaanka u ah dhammaan barnaamijyada xakamaynta DR-TB^[⁴^].

Qiyaasta Farsamada

Kaydinta	≤-18℃
Nolosha shelf	12 bilood
Nooca Tuunada	Tusaalaha xaakada, Dhaqanka Adag (LJ Dhexdhexaad), Dhaqanka dareeraha ah (Medium MGIT)
CV	<5.0%
LoD	LoD ee xirmada lagu ogaanayo qaaxada Mycobacterium waa 10 bakteeriya/ml;LoD ee qalabka lagu ogaanayo nooca duurjoogta ah ee rifampicin iyo nooca mutant waa 150 bakteeriya/mL; LoD ee xirmada lagu ogaanayo nooca duurjoogta ah ee isoniazid iyo nooca mutant waa 200 bakteeriyada/ml.
Gaar ahaan	1) Ma jiro falcelin iskutallaab ah marka la isticmaalayo qalabka si loo ogaado genomic DNA (500ng), noocyada kale ee 28 ee cudurada neef-mareenka, iyo 29 nooc oo ah mycobacteria non-tuberculous (sida ku cad shaxda 3).2) Ma jiro falcelin iskutallaab ah marka la isticmaalayo xirmada si loo ogaado goobaha isbeddelka ee hiddo-wadaha kale ee daawada u adkaysta ee rifampicin iyo isoniazid xasaasiga ah Mycobacterium tuberculosis (sida ku cad shaxda 4).3) Walxaha faragelinta caadiga ah ee shaybaarada la tijaabinayo, sida rifampicin (9mg/L), isoniazid (12mg/L), ethambutol (8mg/L), amoxicillin (11mg/L), oxymetazoline (1mg/L), mupirocin (20mg/L), pyrazinamide), (45mg/Land) (20mg/L) daawooyinka, wax saameyn ah kuma laha natiijooyinka baaritaanka xirmada.
Qalabka lagu dabaqi karo	SLAN-96P Nidaamyada PCR-Waqtiga-dhabta ah (Hongshi Medical Technology Co., Ltd.), BioRad CFX96 Nidaamka PCR-waqtiga-dhabta ah