Mycobacterium tuberculosis Nucleic Acid iyo Rifampicin (RIF), Isoniazid Resistance (INH)

Sharaxaad Gaaban:

Alaabtani waxay ku habboon tahay in la ogaado tayada Mycobacterium tuberculosis DNA ee shaybaarada xaakada bini'aadamka ee vitro, iyo sidoo kale isbeddelka homozygous ee gobolka 507-533 amino acid codon (81bp, gobolka go'aaminta iska caabinta rifampicin) ee hidda-socodka rpoB ee keena qaaxada Mycobacterium iska caabin rifampicin.

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HWTS-RT147 Mycobacterium Tuberculosis Nucleic Acid iyo Rifampicin (RIF), Xirmada Wax-iska-caabbinta Isoniazid (INH)

Epidemiology

Mycobacterium tuberculosis, wax yar sida Tubercle Bacillus (TB), waa bakteeriyada keenta cudurka qaaxada.Waqtigan xaadirka ah, daawooyinka sida caadiga ah loo isticmaalo safka koowaad ee ka hortagga qaaxada waxaa ka mid ah isoniazid, rifampicin iyo ethambutol, iwm. Si kastaba ha ahaatee, isticmaalka khaldan ee daawooyinka ka hortagga qaaxada iyo sifooyinka qaab dhismeedka gidaarka unugyada qaaxada mycobacterium, qaaxada mycobacterium waxay samaysaa caabbinta daawada daawooyinka ka hortagga qaaxada, taas oo keeneysa caqabado halis ah ka hortagga iyo daaweynta qaaxada.

Kanaalka

Magaca Bartilmaameedka			Wariye	Quencher
Reaction BufferA	Reaction BufferB	Reaction BufferC	Wariye	Quencher
rpoB 507-514	rpoB 513-520	IS6110	FAM	Midna
rpoB 520-527	rpoB 527-533	/	CY5	Midna
/	/	Xakamaynta gudaha	HEX (VIC)	Midna

Reaction BufferD	Wariye	Quencher
Gobolka dhiirigeliyaha InhA -15C>T, -8T>A, -8T>C	FAM	Midna
KatG 315 codon 315G>A,315G>C	CY5	Midna
Gobolka horumariyaha AhpC -12C>T, -6G>A	ROX	Midna

Qiyaasaha Farsamada

Kaydinta	≤-18℃
Nolosha shelf	12 bilood
Nooca Tuunada	xaako
CV	≤5.0%
LoD	LoD ee qaaxada mycobacterium tixraaca qaranku waa 50 bakteeriya/mL.LoD ee nooca duurjoogta ah ee u adkaysta rifampicin tixraaca qaranka waa 2×10³bakteeriyada/ml, iyo LoD nooca mutant waa 2×10³bakteeriyada/ml.LoD ee bakteeriyada isoniazid u adkaysata ee nooca duurjoogta ah waa 2x10³bakteeriyada/ml, iyo LoD ee bakteeriyada mutant waa 2x10³bakteeriyada/ml.
Gaar ahaan	Natiijooyinka tijaabada iskutallaabtu waxay muujiyeen in aysan jirin falcelin iskutallaab ah oo lagu ogaanayo genome-ka aadanaha, kuwa kale ee aan ahayn tiibishada mycobacteria iyo cudur-sidaha oof wareenka ee xirmadan leh;Ma jirin falcelin isdhaafsi ah oo laga helay goobaha isbeddelka hiddo-wadaha kale ee daawada u adkaysta ee qaaxada Mycobacterium ee nooca duurjoogta ah.
Qalabka lagu dabaqi karo	SLAN-96P Nidaamyada PCR-Waqtiga-dhabta ah (Hongshi Medical Technology Co., Ltd.), BioRad CFX96 Nidaamka PCR-waqtiga-dhabta ah, Farsamada Hangzhou Bioer ee QuantGene 9600 Nidaamka PCR-waqtiga-dhabta ah, QuantStudio^®5 Nidaamka PCR-waqtiga-dhabta ah.