Mycobacterium Cudulosis nucleic acid iyo rifampicin (RIF), iska caabbinta (INH)

Mycobacterium Cudulosis nucleic acid iyo rifampicin (RIF), iska caabin (INH) muuqaalka muuqaalka

Sharraxaadda Gaaban:

Qalabkan waxaa loo isticmaalaa ogaanshaha tayada leh ee cilmiga fiilada ee cudurka 'mycobacterium qaaxada' DNA 'ee xaakada aadanaha, dhaqanka adag (LJ dhexdhexaad), dheecaanka dareeraha (MGIT), iyo isbadalka ah ee gobolka caanka ah ee aminon (81bp) , Refafampicin Resistance ee go'aaminta gobolka) ee Hange ee RPOB-ga ee cudurka Myscobacterium qaaxada Cadaadiska Rifampicin, iyo sidoo kale isbedelada goobaha isbadalka ugu weyn ee cudurka 'mycobacterium qaaxada is-caabbinta' mycobazulosisisisisisis ', ayaa waxay siisaa gargaar ogaanshaha cudurka' mypobbostrosisisisisisis ', waxayna ogaanaysaa in la ogaado hidda-wadaha ee' Mycobazulosis cudurka 'mypobampium cudurka' mypobboskin cudurka 'mypobboskin cudurka' mypobboskin cudurka 'mypobboskin cudurka' mypobampisisis qaaxada 'viferampicin' iyo Isoniazid, kaas oo ka caawiya fahamka iska caabinta daroogada Cudurka Mycobacterium qaaxada ayaa bukaanku ku dhacay.

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Hwts-rt147 mycobacterium qaaxada acid acid iyo rifampicin (rif), (INH),

Nadiifin

Cudurka qaaxada Mycobacteration, waqti yar sida qaaxada (TB), waa bakteeriyada cudurka qaaxada ee sababa qaaxada, oo hadda, daroogada lidka-tiirarka ee ugu horreeya ee loo isticmaalo "waxaa ka mid ah Isoniazid, Rifampicin iyo Ethambutol, iwm^[1]. Si kastaba ha noqotee, isticmaalka khaldan ee dawooyinka lidka ku ah cudurka qaaxada iyo astaamaha qaab dhismeedka derbiga ee mysbobacterium qaaxada lafteeda, qaaxada Mycobacosis-ka ayaa soo saaray caabbinta dawooyinka ka hortagga cudurka qaaxada, iyo qaab gaar ah oo khatar ah waa qaaxada u adkeysta cudurka qaaxada (Mycobalor). TB), oo u adkaysta labada daroogada ugu badan uguna waxtarka badan, Rifampicin iyo Isoniazid^[2].

Dhibaatada caabbinta dawooyinka qaaxada ee ka jira oo ka jira dhammaan waddamada ay sahamisay yaa. Si loo bixiyo qorshayaal daaweyn oo sax ah oo loogu talagalay bukaannada qaaxada, waxaa lagama maarmaan ah in la ogaado caabbinta daroogada la-dagaallanka qaaxada, gaar ahaan caabbinta 'Rifampicin', kaas oo noqday tallaabo-tallaabo oo ka hortaga cudurka ah, kaas oo noqday tallaabo-tallaabo cudur oo lagu taliyay cidda ku daaweynta cudurka qaaxada^[3]. In kasta oo daahfurka cadaadiska rifampicin ay ku dhowdahay daahfurka MDR-TB, oo keliya ogaanshaha caabbinta rifazicin-ka ah ee diidka ah (u adkeysi u leh rifampicin) iyo rifazicin-ka dina-u-adkeysta (dareenka Isoniazid) laakiin iska caabbinta Rifampicin), oo u horseedi karta bukaannada lagu daweeyo si aan macquul ahayn regimens. Sidaa darteed, Isoniazid iyo Iitapampicictions Resistampance Revidence waa shuruudaha ugu yar ee lagama maarmaanka u ah dhammaan barnaamijyada xakamaynta DRB TB-TB^[⁴^].

Cayayaanka farsamada

Bakhaar	≤-18 ℃
Shelf-Life	12 bilood
Nooca muunadda	Sample Muunada, Dhaqanka adag (LJ Dhexdhexaad), Dhaqanka dareeraha ah (MGIT)
CV	<5.0%
Dhigid	Lod ee xirmada ku saabsan ogaanshaha cudurka 'mycobacterium qaaxada' waa 10 bakteeri / ml;Lod ee xirmada ee ku saabsan nooca rifampampicin nooca duurjoogta ah iyo nooca jilicsan waa 150 bakteeriyada / ml; Lod ee xirmada ee loo baahan yahay garashada Isoniazid nooca duurjoogta ah iyo nooca la mutadaaciyaa waa 200 bakteeriyada / ML.
Si gaar ah	1) Ma jiro ficil celin iskutallaab ah marka la isticmaalo xirmada si loo ogaado DNA-da GNA-da ee aadanaha (500ng), 28 nooc oo ka mid ah cudurada neef-mareenka, iyo 29 nooc oo ah my -Bootullaahihi Ciidda (sida lagu muujiyey jadwalka 3).2) Ma jirto ficil celinta iskutallaabta marka la adeegsanayo xirmada si loo ogaado goobaha isbadalka ee loogu talagalay hiddo-wadayaasha kale ee ugu adkaysata daroogada ee Rifampampin iyo Isoniazid xasaasiga ah cudurka 'mycobazosium' (sida lagu muujiyey shaxda 4).3) Waxyaabaha faragalinta caanka ah ee muunadaha la isku daro ee muunadaha la tijaabinayo, sida Rifampicin (9mg / L), Isoniazud (12mg / L), Amoxbutol (L), Oxytazoline (1MG / L), MUPROCIN (20mg / L), pyrazinamide (45mg / L), zanamivir (0.5MG / L), Dexametsosone (20mg / L) Daroogada, wax saameyn ah kuma lahan natiijooyinka baaritaanka xirmada.
Aaladaha lagu dabaqi karo	Nidaamyada Slan-96p ee waqtiga-labaad ee PCR (tikniyoolajiyadda caafimaadka ee Hongshi) CO., LTD.), Biorad Cfx96 Nidaamka waqtiga-dhabta ah ee PCR