Mycobacterium Tuberculosis Asiidh Nucleic iyo Rifampicin (RIF), Iska caabin (INH)

Mycobacterium Tuberculosis Nucleic Acid iyo Rifampicin (RIF)，Iska caabinta (INH) Sawirka Lagu Soo Bandhigay

Sharaxaad Gaaban:

Qalabkan waxaa loo isticmaalaa ogaanshaha tayada ee DNA-da qaaxada Mycobacterium ee candhuufta aadanaha, dhaqanka adag (LJ Medium) iyo dhaqanka dareeraha (MGIT Medium), dareeraha laamiga ee bronchial, iyo isbeddellada ku yimaada gobolka codon amino acid 507-533 (81bp, gobolka go'aaminta iska caabbinta rifampicin) ee hidda-wadaha rpoB ee iska caabbinta qaaxada Mycobacterium rifampicin, iyo sidoo kale isbeddellada ku dhaca goobaha ugu muhiimsan ee qaaxada Mycobacterium isoniazid. Waxay kaa caawineysaa ogaanshaha caabuqa qaaxada Mycobacterium, waxayna ogaataa hidda-wadaha iska caabbinta ugu weyn ee rifampicin iyo isoniazid, taas oo ka caawisa fahamka iska caabbinta daawada ee qaaxada Mycobacterium ee uu ku dhacay bukaanka.

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Qalabka Ogaanshada ee HWTS-RT147 Mycobacterium Tuberculosis Nucleic Acid iyo Rifampicin (RIF), (INH) (Qalooca Dhalaalka)

Cudurrada Faafa

Qaaxada Mycobacterium, oo dhawaan loo yaqaan Tubercle Bacillus (TB), waa bakteeriyada keenta cudurka qaaxada, haddana, daawooyinka ugu horreeya ee ka hortagga qaaxada ee loo isticmaalo waxaa ka mid ah isoniazid, rifampicin iyo ethambutol, iwm.^[1]Si kastaba ha ahaatee, sababtoo ah isticmaalka khaldan ee daawooyinka ka hortagga qaaxada iyo astaamaha qaab-dhismeedka derbiga unugyada ee qaaxada mycobacterium lafteeda, qaaxada mycobacterium waxay horumarisay iska caabbinta daawada ee daawooyinka ka hortagga qaaxada, qaab gaar ah oo khatar ah waa qaaxada u adkaysata daawooyinka badan (MDR-TB), taas oo u adkaysata labada daawo ee ugu badan uguna waxtarka badan, rifampicin iyo isoniazid.^[2].

Dhibaatada ka dhalata iska caabbinta daawada qaaxada waxay ka jirtaa dhammaan waddamada ay WHO sahaminayso. Si loo bixiyo qorshayaal daaweyn oo sax ah oo loogu talagalay bukaanada qaaxada, waxaa lagama maarmaan ah in la ogaado iska caabbinta daawooyinka ka hortagga qaaxada, gaar ahaan iska caabbinta rifampicin, taasoo noqotay tallaabo lagu ogaanayo oo ay WHO ku talisay daaweynta qaaxada.^[3]In kasta oo helitaanka iska caabbinta rifampicin ay ku dhowdahay inay la mid tahay helitaanka MDR-TB, ogaanshaha iska caabbinta rifampicin oo keliya ayaa iska indha tiraysa bukaanada qaba INH-ga mono-resistant (oo tilmaamaya iska caabbinta isoniazid laakiin u nugul rifampicin) iyo rifampicin mono-resistant (xasaasiyad loo qabo isoniazid laakiin u adkaysata rifampicin), taas oo horseedi karta bukaanada loo maro nidaamyo daaweyn oo aan macquul ahayn oo bilow ah. Sidaa darteed, tijaabooyinka iska caabbinta isoniazid iyo rifampicin waa shuruudaha ugu yar ee lagama maarmaanka ah ee dhammaan barnaamijyada xakamaynta DR-TB.^[⁴^].

Halbeegyada Farsamada

Kaydinta	≤-18℃
Muddada khaanadaha	12 bilood
Nooca Muunada	Muunadda Xaakada, Dhaqanka Adag (LJ Dhexdhexaad), Dhaqanka Dareeraha ah (MGIT Dhexdhexaad)
CV	<5.0%
LoD	LoD ee qalabka lagu ogaanayo qaaxada Mycobacterium waa 10 bakteeriya/mL;LoD-ga qalabka lagu ogaanayo nooca duurjoogta ah ee rifampicin iyo nooca mutant waa 150 bakteeriya/mL; LoD-ga qalabka lagu ogaanayo nooca duurjoogta ah ee isoniazid iyo nooca mutant waa 200 bakteeriya/mL.
Gaar ahaanshaha	1) Ma jirto wax falcelin isdhaafsi ah marka la isticmaalayo qalabka si loo ogaado DNA-da hidde-sideyaasha aadanaha (500ng), 28 nooc oo kale oo ah jeermisyada neefsashada, iyo 29 nooc oo ah mycobacteria aan qaaxada lahayn (sida ku cad Shaxda 3).2) Ma jirto wax falcelin isdhaafsi ah marka la isticmaalayo qalabka si loo ogaado goobaha isbeddelka ee hiddo-sideyaasha kale ee daawada u adkaysta ee rifampicin iyo qaaxada Mycobacterium ee xasaasiga ah ee isoniazid (sida ku cad Shaxda 4).3) Daawooyinka faragelinta badan ee ku jira muunadaha la baarayo, sida rifampicin (9mg/L), isoniazid (12mg/L), ethambutol (8mg/L), amoxicillin (11mg/L), oxymetazoline (1mg/L), mupirocin (20mg/L), pyrazinamide (45mg/L), zanamivir (0.5mg/L), dexamethasone (20mg/L), wax saameyn ah kuma yeelanayaan natiijooyinka baaritaanka qalabka.
Qalabka Khuseeya	Nidaamyada PCR-ga Waqtiga-dhabta ah ee SLAN-96P (Hongshi Medical Technology Co., Ltd.), Nidaamka PCR-ga ee Waqtiga-dhabta ah ee BioRad CFX96