Mycobacterium Tuberculosis iska caabinta Rifampicin
Magaca alaabta
HWTS-RT074A-Mycobacterium Tuberculosis Rifampicin Qalabka Ogaanshaha Iska-caabbinta (Fluorescence PCR)
Epidemiology
Rifampicin ayaa si weyn loogu isticmaalay daawaynta bukaanada qaaxada sambabada tan iyo dabayaaqadii 1970-meeyadii, waxayna leedahay saameyn weyn.Waxay ahayd doorashada koowaad ee lagu soo gaabiyo daaweynta kemotherabi ee bukaannada qaaxada sambabada.Iska caabbinta Rifampicin waxaa badanaa sababa isbeddelka hidda-wadaha rpoB.Inkasta oo dawooyinka cusub ee ka hortagga qaaxada ay si joogto ah u soo baxayaan, iyo waxtarka bukaan-socodka ee bukaannada qaaxada sambabada ayaa sidoo kale sii waday inay soo roonaato, weli waxaa jira la'aanta daawooyinka ka hortagga qaaxada, iyo ifafaalaha isticmaalka daroogada aan macquul ahayn ee bukaan-socodka waa mid aad u sarreeya.Sida iska cad, qaaxada Mycobacterium ee bukaanada qaba qaaxada sambabada gabi ahaanba lama dili karo waqti ku haboon, taasoo aakhirka keenta in qofka bukaanka ah uu jidhkiisa iska caabiyo darajooyin kala duwan, siina dheerayso muddada cudurka, kordhisana khatarta dhimashada bukaanka.Qalabkani waxa uu ku habboon yahay ogaanshaha kaaliyaha ee caabuqa qaaxada Mycobacterium iyo ogaanshaha hiddasidaha iska caabinta rifampicin, taas oo waxtar u leh in la fahmo iska caabbinta daawada qaaxada mycobacterium ee uu buka buka, iyo in la siiyo habab caawimo ah oo lagu hago dawooyinka kiliinikada.
Epidemiology
Magaca Bartilmaameedka | Wariye | Quencher | ||
Reaction BufferA | Reaction BufferB | Reaction BufferC | ||
rpoB 507-514 | rpoB 513-520 | IS6110 | FAM | Midna |
rpoB 520-527 | rpoB 527-533 | / | CY5 | Midna |
/ | / | Xakamaynta gudaha | HEX (VIC) | Midna |
Qiyaasaha Farsamada
Kaydinta | ≤-18℃ Madow |
Nolosha shelf | 9 bilood |
Nooca Tuunada | xaako |
CV | <5 |
LoD | nooca duurjoogta ah ee rifampicin: 2x103bakteeriyada/ml mutant homozygous: 2x103bakteeriyada/ml |
Gaar ahaan | Waxay ogaataa qaaxada mycobacterium-ka duurjoogta ah iyo goobaha isku-bedelka ee hidde-sideyaasha kale ee iska caabbinta daroogada sida katG 315G>C\A, InhA-15C>T, natiijooyinka baaritaanku waxay muujinayaan wax iska caabin ah oo loo yaqaan 'rifampicin', taas oo macnaheedu yahay inaysan jirin falcelin iskutallaab ah. |
Qalabka lagu dabaqi karo: | SLAN-96P Nidaamyada PCR-Waqtiga-dhabta ah (Hongshi Medical Technology Co., Ltd.) |