Mycobacterium Tuberculosis Aashitada Nucleic iyo Rifampicin iska caabbinta
Magaca alaabta
Qalabka Ogaanshada Iska caabinta ee HWTS-RT074B-Mycobacterium Tuberculosis Nucleic Acid iyo Rifampicin (Qalooca Dhalaalka)
Shahaadada
CE
Cudurrada Faafa
Qaaxada Mycobacterium , waqti yar ka dib markii Tubercle Bacillus, TB, ay tahay bakteeriyada keenta cudurka qaaxada. Waqtigan xaadirka ah, daawooyinka ka hortagga qaaxada ee safka hore ee loo isticmaalo waxaa ka mid ah isoniazid, rifampicin iyo hexambutol, iwm. Daawooyinka ka hortagga qaaxada ee safka labaad waxaa ka mid ah fluoroquinolones, amikacin iyo kanamycin, iwm. Daawooyinka cusub ee la sameeyay waa linezolid, bedaquiline iyo delamani, iwm. Si kastaba ha ahaatee, sababtoo ah isticmaalka khaldan ee daawooyinka ka hortagga qaaxada iyo astaamaha qaab-dhismeedka derbiga unugyada ee qaaxada mycobacterium, qaaxada mycobacterium waxay horumarisaa iska caabbinta daawada ee daawooyinka ka hortagga qaaxada, taas oo keenta caqabado halis ah oo ku yimaada ka hortagga iyo daaweynta qaaxada.
Rifampicin waxaa si weyn loogu isticmaalay daaweynta bukaanada qaaxada sambabada tan iyo dabayaaqadii 1970-meeyadii, waxayna leedahay saameyn muhiim ah. Waxay ahayd doorashadii ugu horreysay ee lagu soo gaabiyo kiimoterabiga bukaanada qaaxada sambabada. Iska caabbinta Rifampicin waxaa inta badan sababa isbeddelka hidda-wadaha rpoB. In kasta oo daawooyinka cusub ee ka hortagga qaaxada ay si joogto ah u soo baxayaan, waxtarka caafimaad ee bukaanada qaaxada sambabadana ay sii wanaajisay, haddana waxaa jira la'aan qaraabada ah oo daawooyinka ka hortagga qaaxada ah, iyo dhacdada isticmaalka daroogada aan macquulka ahayn ee kiliinikada waa mid aad u sarreysa. Sida iska cad, qaaxada Mycobacterium ee bukaanada qaba qaaxada sambabada si buuxda looma dili karo waqtigeeda, taasoo ugu dambeyntii keenta heerar kala duwan oo iska caabbinta daawada ah oo ku jira jirka bukaanka, waxay sii dheereysaa muddada cudurka, waxayna kordhisaa khatarta dhimashada bukaanka.
Kanaal
| Kanaal | Kanaalada iyo Fluorophores | Kaydka Falcelinta A | Kaydka Falcelinta B | Kaydka Falcelinta C |
| Kanaalka FAM | Weriye: FAM, Quencher: Midna | rpoB 507-514 | rpoB 513-520 | 38KD iyo IS6110 |
| Kanaalka CY5 | Weriye: CY5, Quencher: Midna | rpoB 520-527 | rpoB 527-533 | / |
| Kanaalka HEX (VIC) | Weriye: HEX (VIC), Quencher: Midna | Xakamaynta gudaha | Xakamaynta gudaha | Xakamaynta gudaha |
Halbeegyada Farsamada
| Kaydinta | ≤-18℃ Mugdi ku jira |
| Muddada khaanadaha | 12 bilood |
| Nooca Muunada | Xaako |
| CV | ≤5.0% |
| LoD | Qaaxada Mycobacterium 50 bakteeriya/mL Nooca duurjoogta ah ee u adkaysta rifampicin: 2x103bakteeriyada/mL mutant homozygous ah: 2x103bakteeriyada/mL |
| Gaar ahaanshaha | Waxay ogaataa qaaxada nooca duurjoogta ah ee mycobacterium iyo goobaha isbeddelka ee hiddo-sideyaasha kale ee iska caabbinta daawada sida katG 315G>C\A, InhA-15C> T, natiijooyinka baaritaanku ma muujinayaan wax iska caabin ah oo ku wajahan rifampicin, taasoo la macno ah inaysan jirin wax falcelin isdhaafsi ah. |
| Qalabka Khuseeya: | Nidaamyada PCR-ga Waqtiga-dhabta ah ee SLAN-96P Nidaamka PCR-ga ee Waqtiga-dhabta ah ee BioRad CFX96 Nidaamka PCR-ga ee Waqtiga-dhabta ah ee LightCycler480® |
Socodka Shaqada

Haddii aad isticmaasho Qalabka Guud ee Macro & Micro-Test DNA/RNA (HWTS-3019-50, HWTS-3019-32, HWTS-3019-48, HWTS-3019-96) (oo loo isticmaali karo Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006C, HWTS-3006B)) ama Macro & Micro-Test Viral DNA/RNA Column (HWTS-3022-50) oo ay samaysay Jiangsu Macro & Micro-Test Med-Tech Co., Ltd. si aad u soo saarto, ku dar 200μL oo ah muunadda Xakamaynta Togan, xakamaynta taban iyo muunadda xaakada la farsameeyay si loogu tijaabiyo si isku xigta, oo ku dar 10μL oo ah xakamaynta gudaha si gaar ah muunadda Xakamaynta Togan, xakamaynta taban iyo muunadda xaakada la farsameeyay si loo tijaabiyo, tallaabooyinka xigana waa in si adag loo sameeyaa iyadoo la raacayo tilmaamaha soo saarista. Mugga muunadda la soo saaray waa 200μL, mugga dheecaanka lagu taliyayna waa 100μL.








