Mycobacterium qaaxada nucleic acid iyo iska caabbinta rifamprampicinta
Magaca wax soo saarka
Hwts-rt074b-mycobacterium qaaxada nucleic acid iyo kaydinta ogaanshaha ee diirinta ee reifampic conce (qalooca dhalaalaya)
Shahaado
CE
Nadiifin
Cudurka qaaxada Mycobacterium, waxyar sida qaaxada, TB, waa bakteeriyada cudurka qaaxada ee sababa qaaxada. Waqtiga xaadirka ah, daroogada ugu horreeya ee loo yaqaan 'the daroogada ugu horreeya ee loo isticmaalo' 'daroogooyinka la taaban karo ee loo yaqaan' ka-hortagga 'vifarampicin' iyo Hexampbutol, iwm. Daawooyinka ka hortagga cudurka qaaxada, iwm. . Si kastaba ha noqotee, sababta oo ah isticmaalka khaldan ee Daawooyinka cudurka qaaxada iyo astaamaha qaab dhismeedka derbiga ee Mycobacterium Cudurka qaaxada, qaaxada Mycobacterium Cudurka qaaxada, tiirarka Mycobacteration Cudurka ayaa kobcisa iska caabbinta daroogada ee daroogada ka hortagga cudurka qaaxada, oo caqabado culus u keenaya ka hortagga iyo daaweynta qaaxada.
Rifampicin waxaa si weyn loogu adeegsaday daaweynta bukaannada qaaxada sambabka ee sambabada tan iyo dabayaaqadii 1970-yadii, waxayna saameyn weyn ku leedahay. Waxay ahayd doorashada ugu horreysa ee ka soo gaabinaysa kiimiko kuayaha bukaan socodka sambabada sambabada. Caabbilooninta Rifampicicin waxaa inta badan sababa isbadalka wadaha RPOB. In kasta oo daroogada cusub ee ka hortagga cudurka qaaxada ay si joogto ah u soo baxaan, iyo waxtarka caafimaad ee bukaan-socodka bukaan-socodka ayaa sidoo kale sii waday in la wanaajiyo daroogada qaaxada, iyo ifafaale loo isticmaalo maandooriyaha aan macquul ahayn ee kiliinikada ayaa ah mid aad u sarreeya. Sida iska cad, qaaxada Mycobacterosis ee bukaanka qaba cudurka qaaxada sambabka laguma dili karo waqti ku habboon, oo ay ugu dambeyntii horseedda iska caabbinta daroogada, waxay kordhisaa halista dhimashada bukaanka.
Kanaal
| Kanaal | Kanaalada iyo flulorophores | Falcelin jawaab celin a | Dib-u-soo-celinta Buffer B | Firfircoonaanta Rabshadaha C |
| Kanaalka caanka ah | Wariyaha: FAM, Quinchen: Midna | RPOB 507-514 | RPOB 513-520 | 38kd iyo IS6110 |
| Ch5 Channel | Wariyaha: Cy5, Qushrur: Midna | RPOB 520-527 | RPOB 527-533 | / |
| Hex (vic) kanaalka | Wariyaha: Hex (Vic), Quinchen: Midna | Kontoroolka gudaha | Kontoroolka gudaha | Kontoroolka gudaha |
Cayayaanka farsamada
| Bakhaar | -18 ℃ mugdi |
| Shelf-Life | 12 bilood |
| Nooca muunadda | Munaafo dad |
| CV | ≤5.0% |
| Dhigid | Mycobacterium Cuberculosis 50 bakteeriyada / ml Nooca duurjoogta ah ee Rifampicin-ka3Bakteeriyada / ml homozygous paters: 2x103Bakteeriyada / ml |
| Si gaar ah | Waxay ogaataa cudurka 'mycobacterium-ka nooca' Mycobactesium nooca loo yaqaan 'Mycobalosium nooca' iyo goobaha isbadalka ee loogu talagalay hidda-wadaha caadiga ah sida Katg 315g> C \ A, natiijada natiijada baaritaanka ma muujinayaan caabbinta rifampicin, taas oo macnaheedu yahay inaysan jirin wax-ka-fal-celin. |
| Aaladaha lagu dabaqi karo: | Nidaamyada 'Slacan-96p' ee waqtiga dhabta ah ee PCR Biorad Cfx96 Nidaamka waqtiga-dhabta ah ee PCR Nalalka Nalalka480® Nidaamka PCR-ka waqtiga-dhabta ah |
Socodka shaqada
Haddii aad isticmaasho maacuulka guud ee DNA / RNA (HWTS-3019-50-59-32, hwts-3019-48, hwts-3019-96) Soosaaraha Nucleic acid (hwts-3006c, hwts-3006b)) ama macro & Imtixaanka Micro-Plac Dana / RNA Sxarta (HWTS-3022-50) Jaangsu Macro & Teast Micro-Tech Co. Tech-Tech-Tech, xakamaynta taranka, xambaarsan taban oo la farsameeyay isku xigxiga, oo ku dar 10μl kontoroolka gudaha si gooni gooni ah gacanta ugu sareysa, xakamaynta taban iyo shaashadda xaakada la socodsiiyay, iyo tillaabooyinka xiga waa inay ahaadaan si adag loo fuliyay iyadoo loo eegayo tilmaamaha soo saarista. Mugga muunad ee muunad la soo saaray waa 200μl, oo mugga ka-wareejinta lagu talinayo waa 100μl.
