Cudurada Neefsashada ee La isku daray

Sharaxaad Gaaban:

Xirmadan waxa loo isticmaalaa in lagu ogaado tayada cudur-sidaha neef-mareenka ee ku jira aashitada nucleic-ka ah ee laga soo saaro muunadaha suufka oropharyngeal ee bini'aadamka.

Qaabkan waxa loo isticmaalaa in lagu ogaado tayada 2019-nCoV, fayraska hargabka A, fayraska hargabka B iyo fayraska syncytial nucleic acids ee neefsiga ee muunado suuf oropharyngeal.


Faahfaahinta Alaabta

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Magaca alaabta

HWTS-RT158A Qalabaynta Cudurada Neefsiga ee Isku-dhafka ah ee Qalabka Garashada (Fluorescence PCR

Shahaado

CE

Epidemiology

Cudurka Corona Virus 2019, oo loo yaqaan'COVID 19', waxa loola jeedaa sambabada oo uu keeno caabuqa 2019-nCoV.2019-nCoV waa coronavirus ka tirsan hiddaha β.COVID-19 waa cudur faafa oo neef-mareenka oo ba'an, dadkuna guud ahaan waa u nugul yihiin.Waqtigan xaadirka ah, isha infekshanku waa inta badan bukaanada uu haleelay 2019-nCoV, iyo dadka asymptomatic-ka qaba ayaa sidoo kale noqon kara isha caabuqa.Iyada oo ku saleysan baaritaanka cudurrada faafa ee hadda, muddada soo-kabashada waa 1-14 maalmood, badiyaa 3-7 maalmood.Qandho, qufac qallalan iyo daal ayaa ah calaamadaha ugu waaweyn.Bukaanno dhowr ah ayaa lahaa calaamado ay ka mid yihiin ciriiriga sanka, diif, dhuun xanuun, myalgia iyo shuban, iwm.

Hargabka, oo loo yaqaan "flu", waa cudur faafa oo neef-mareenka oo ba'an oo uu keeno fayraska hargabka.Aad buu u faafayaa.Inta badan waxa lagu kala qaadaa qufaca iyo hindhisada.Caadiyan waxay soo baxdaa guga iyo jiilaalka.Fayrasyada hargabku waxay u qaybsan yihiin hargab A (IFV A), hargabka B (IFV B), iyo Hargabka C (IFV C) saddex nooc, dhammaan waxay ka tirsan yihiin fayras dhegdheg ah, waxay sababaan cudurrada bini'aadamka inta badan fayraska A iyo B, waa hal mar. fayraska RNA oo kala go'ay.Hargabka A waa caabuq neef-mareenka oo degdeg ah, oo ay ku jiraan H1N1, H3N2 iyo noocyo kale oo hoose, kuwaas oo u nugul isbeddelka iyo dillaaca adduunka oo dhan."Shift" waxaa loola jeedaa beddelka fayraska hargabka A, taasoo keentay soo bixitaanka fayraska cusub "subtype".Fayrasyada hargabka B waxa loo qaybiyaa laba abtirsiimo, Yamagata iyo Victoria.Fayraska hargabka B waxa uu leeyahay oo kaliya fayraska antigenic, waxana uu ka baxsadaa ilaalinta iyo baabi'inta habka difaaca bini'aadamka iyada oo loo marayo isbeddelkiisa.Si kastaba ha ahaatee, xawaaraha kobaca ee fayraska hargabka B wuu ka gaabiyaa kan fayraska hargabka A.Fayraska hargabka B wuxuu kaloo keeni karaa caabuqyada neef-mareenka ee bani'aadamka wuxuuna u horseedi karaa cudurro faafa.

Fayraska isku dhafan ee neefsashada (RSV) waa fayras RNA, oo ka tirsan qoyska paramyxoviridae.Waxaa lagu kala qaadaa dhibco hawo ah iyo xiriir dhow waana cudur keenaha ugu weyn ee caabuqa mareenka neef-mareenka hoose ee dhallaanka.Dhallaanka uu ku dhacay RSV waxaa laga yaabaa inay yeeshaan bronchiolitis daran iyo oof-wareen, kuwaas oo la xiriira neefta carruurta.Dhallaanku waxay leeyihiin calaamado aad u daran, oo ay ku jiraan qandho sare, rhinitis, pharyngitis iyo laryngitis, ka dibna bronkiolitis iyo oof-wareen.Dhawr carruur ah oo buka ayaa ku adkaan kara otitis media, pleurisy iyo myocarditis, iwm. Infekshanka mareenka sare ee neef-mareenka waa calaamadda ugu weyn ee caabuqa ee dadka waaweyn iyo carruurta ka weyn.

Kanaalka

FAM SARS-CoV-2
VIC(HEX) RSV
CY5 IFV A

ROX

IFV B

Quasar 705

Xakamaynta Gudaha

Qiyaasaha Farsamada

Kaydinta

-18℃

Nolosha shelf 12 bilood
Nooca Tuunada Oropharyngeal suuf
Ct ≤38
LoD 2019-nCoV: 300Koobi/ml

Fayraska Hargabka A/Fayraska Hargabka B/Fayraska la midka ah ee neefsiga: 500 koobi/mL

Gaar ahaan a) Natiijooyinka falcelinta isdhaafsiga ayaa muujinaya in aysan jirin falcelin iskutallaab ah oo udhaxeysa xirmada iyo coronavirus bini aadamka SARSr-CoV, MERSr-CoV, HCoV-OC43, HCoV-229E, HCoV-HKU1, HCoV-NL63, fayraska parainfluenza nooca 1, 2, 3, rhinovirus A, B, C, chlamydia pneumoniae, human metapneumovirus, enterovirus A, B, C, D, fayraska epstein-barr, fayraska jadeecada, fayraska cytomegalo, rotavirus, norovirus, fayraska parotitis, varicella-zoster virus, legionella, xiiq-dheerta, haemophilus influenzae, staphylococcus aureus, streptococcus pneumoniae, streptococcus pyogenes, klebsiella pneumoniae, qaaxada mycobacterium, qiiqa aspergillus, candida albicans, candida glabrata, pneumoniic acid jiro iyo kryptococcussychistikis.

b) Awoodda ka-hortagga faragelinta: dooro mucin (60mg/mL), 10% (v/v) oo dhiig ah iyo phenylephrine (2mg/mL), oxymetazoline (2mg/mL), chloride sodium (oo ay ku jiraan kuwa ilaaliya) (20mg/mL) , beclomethasone (20mg/mL), dexamethasone (20mg/mL), flunisolide (20μg/mL), triamcinolone acetonide (2mg/mL), budesonide (2mg/mL), mometasone (2mg/mL), fluticasone (2mg/mL) , histamine hydrochloride (5mg/mL), alfa interferon (800IU/ml), zanamivir (20mg/mL), ribavirin (10mg/mL), oseltamivir (60ng/mL), peramivir (1mg/mL), lopinavir (500mg/mL). Ritonavir (60mg/ml), mupirocin (20mg/mL), azithromycin (1mg/mL), ceftriaxone (40μg/ml), meropenem (200mg/mL), levofloxacin (10μg/ml) iyo tobramycin (0.6mg/mL) ) baaritaanka faragelinta, natiijaduna waxay muujinaysaa in walxaha faragelinta leh ee kor ku xusan aysan lahayn falcelin faragelin ah oo ku saabsan natiijooyinka baaritaanka cudur-sidaha.

Qalabka lagu dabaqi karo BioRad CFX96 Nidaamka PCR-waqtiga-dhabta ah

Rotor-Gene Q 5plex HRM Platform Nidaamka PCR-waqtiga-dhabta ah

Xirmooyinka ogaanshaha Isku-dhafka Cudurada Neefsiga (Fluorescence PCR)

Wadarta Xalka PCR


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