Fayraska Zika
Magaca alaabta
HWTS-FE002 Xirmada ogaanshaha Ashitada Nukliyeerka ee Zika Virus (Fluorescence PCR)
Shahaado
CE
Epidemiology
Fayraska Zika waxaa iska leh genus Flaviviridae, waa fayraska RNA-ga ee togan ee hal-xidhaale leh oo dhexroorkiisu yahay 40-70nm. Waxay leedahay baqshad, oo ka kooban 10794 nucleotides, oo ay ku dhejiso 3419 amino acids. Marka loo eego genotype, waxa loo qaybiyaa nooca Afrika iyo nooca Aasiya. Cudurka fayraska Zika waa cudur faafa oo ba'an oo is-xakameeya oo ay keento fayraska Zika, kaas oo inta badan lagu kala qaado qaniinyada kaneecada Aedes aegypti. Astaamaha caafimaadku waa inta badan qandho, finan, arthralgia ama conjunctivitis, waana dhif dhimasho. Sida laga soo xigtay Ururka Caafimaadka Adduunka, microcephaly dhallaanka iyo Guillain-Barre syndrome (Guillain-Barré syndrome) ayaa laga yaabaa inay la xiriiraan caabuqa fayraska Zika.
Kanaalka
| FAM | Zika virus nucleic acid |
| ROX | Xakamaynta Gudaha |
Qiyaasta Farsamada
| Kaydinta | ≤30℃ & iftiinka laga ilaaliyo |
| Nolosha shelf | 12 bilood |
| Nooca Tuunada | serum cusub |
| Ct | ≤38 |
| CV | <5.0% |
| LoD | 500 koobi/ml |
| Gaar ahaan | Isticmaal qalabka si aad u ogaato shaybaarada serum-ka ee fayraska Zika taban, natiijaduna waa taban. Natiijooyinka baaritaanka faragelinta waxay muujinayaan in marka fiirsashada bilirubin ee serum aysan ka badneyn 168.2μmol/ml, xoojinta haemoglobin ee ay soo saarto hemolysis waa inaysan ka badneyn 130g/L, fiirsashada dufanka dhiigga aan ka badneyn 65mmol/ml, wadarta xoojinta IgG ee serum kama badna 5mg/mL, ma jiro wax saameyn ah oo ku saabsan fayraska dengue. Fayraska Cagaarshowga, Cagaarshowga B, Cagaarshowga C, Fayraska Herpes, Fayraska Equine encephalitis Eastern, Hantavirus, Bunya Virus, Virus-ka West Nile iyo shaybaarada serum genomic ee dadka ayaa loo xushay baaritaanka falcelinta falcelinta, natiijaduna waxay muujineysaa in aysan jirin falcelin udhaxeysa xirmadan iyo cudur-sidaha kor ku xusan. |
| Qalabka lagu dabaqi karo | ABI 7500 Nidaamyada PCR-waqtiga-dhabta ahABI 7500 Nidaamyada PCR-Waqtiga-Dhakhsada leh QuantStudio®5 Nidaamyada PCR-waqtiga-dhabta ah SLAN-96P Nidaamyada PCR-Waqtiga-dhabta ah LightCycler®480 Nidaamka PCR-waqtiga dhabta ah LineGene 9600 Plus-Waqtiga dhabta ah ee PCR Nidaamka ogaanshaha MA-6000-waqtiga-dhabta ah ee cabbirka kulaylka wareegga BioRad CFX96 Nidaamka PCR-waqtiga-dhabta ah BioRad CFX Opus 96 Nidaamka PCR-waqtiga-dhabta ah |
Socodka Shaqada
Doorashada 1.
QIAamp Viral RNA Mini Kit(52904), Soo saarista Asiidhka Nukliyeerka ah ama NadiifintaYDP315-R) ee Tiangen Biotech(Beijing) Co., Ltd.Soo saaristawaa in la soo saaraa iyadoo la raacayo tilmaamaha soo saarista, iyo mugga soo saarista lagu taliyey waa 140 μL iyo mugga sare ee lagu taliyey waa 60 μL.
Doorashada 2.
Makro & Micro-Test Viral DNA/RNA Kit (HWTS-3004-32, HWTS-3004-48,HWTS-3004-96) Soo saarista waa in lagu soo saaraa si waafaqsan tilmaamaha. Mugga muunadda soo saariddu waa 200 μL, mugga elutionka ee lagu taliyeyna waa 80μL.
