Isbeddelka Hidde-sidaha ee Aadanaha ee CYP2C19
Magaca alaabta
Qalabka Ogaanshada Isbeddelka Hidde-sidaha ee HWTS-GE012A-Human CYP2C19 (Fluorescence PCR)
Shahaadada
CE/TFDA
Cudurrada Faafa
CYP2C19 waa mid ka mid ah enzymes-ka muhiimka ah ee dheef-shiid kiimikaadka daawada ee qoyska CYP450. Substrates badan oo gudaha ah iyo qiyaastii 2% daawooyinka kiliinikada ah waxaa dheef-shiid kiimikaadka ku sameeya CYP2C19, sida dheef-shiid kiimikaadka daawooyinka ka hortagga isku-darka platelet-ka (sida clopidogrel), daawooyinka ka hortagga proton-ka (omeprazole), daawooyinka ka hortagga isku-dhafka, iwm. Isbeddellada hidda-wadaha CYP2C19 sidoo kale waxay leeyihiin kala duwanaansho ku saabsan awoodda dheef-shiid kiimikaadka ee daawooyinka la xiriira. Isbeddelladan dhibcaha ah ee *2 (rs4244285) iyo *3 (rs4986893) waxay sababaan luminta dhaqdhaqaaqa ensaymka ee uu qeexay hidda-wadaha CYP2C19 iyo daciifnimada awoodda substrate-ka dheef-shiid kiimikaadka, waxayna kordhiyaan fiirsashada dhiigga, si ay u keenaan falcelinno daroogo oo xun oo la xiriira fiirsashada dhiigga. *17 (rs12248560) waxay kordhin kartaa dhaqdhaqaaqa ensaymka ee uu qeexay hidda-wadaha CYP2C19, soo saarista metabolites-ka firfircoon, waxayna kor u qaadi kartaa joojinta isku-darka platelet-ka waxayna kordhin kartaa khatarta dhiig-baxa. Dadka qaba dheef-shiid kiimikaad gaabis ah oo daroogo ah, qaadashada qiyaaso caadi ah muddo dheer waxay sababi doontaa sun iyo waxyeelooyin halis ah: inta badan dhaawaca beerka, dhaawaca nidaamka dhiigga, dhaawaca habdhiska dhexe ee neerfaha, iwm., taas oo horseedi karta dhimasho kiisaska daran. Sida laga soo xigtay kala duwanaanshaha shaqsiga ee dheef-shiid kiimikaadka daawada ee u dhigma, guud ahaan waxaa loo qaybiyaa afar nooc oo kala duwan, kuwaas oo kala ah dheef-shiid kiimikaad aad u dhakhso badan (UM,*17/*17,*1/*17), dheef-shiid kiimikaad degdeg ah (RM,*1/*1), dheef-shiid kiimikaad dhexdhexaad ah (IM, *1/*2, *1/*3), dheef-shiid kiimikaad gaabis ah (PM, *2/*2, *2/*3, *3/*3).
Kanaal
| FAM | CYP2C19*2 |
| CY5 | CYP2C9*3 |
| ROX | CYP2C19*17 |
| VIC/HEX | IC |
Halbeegyada Farsamada
| Kaydinta | Dareere: ≤-18℃ |
| Muddada khaanadaha | 12 bilood |
| Nooca Muunada | Dhiig cusub oo xinjirowga lidka ku ah EDTA |
| CV | ≤5.0% |
| LoD | 1.0ng/μL |
| Gaar ahaanshaha | Ma jiro wax fal-celin ah oo isku-dhafan oo leh taxane kale oo aad isugu xiran (hidde-side CYP2C9) oo ku jira hiddo-wadaha aadanaha. Isbeddellada goobaha CYP2C19*23, CYP2C19*24 iyo CYP2C19*25 ee ka baxsan baaxadda ogaanshaha ee xirmadan saameyn kuma yeeshaan saameynta ogaanshaha xirmadan. |
| Qalabka Khuseeya | Nidaamyada Bayoolajiga ee La Adeegsaday 7500 Nidaamyada PCR-ga Waqtiga-dhabta ah Nidaamyada Bayoolajiga ee La Adeegsaday 7500 Nidaamyada PCR-ga ee Waqtiga-dhabta ah ee Degdegga ah Nidaamyada PCR-ga ee waqtiga-dhabta ah ee QuantStudio®5 Nidaamyada PCR-ga Waqtiga-dhabta ah ee SLAN-96P Nidaamka PCR-ga ee waqtiga-dhabta ah ee LightCycler®480 Nidaamka Ogaanshaha PCR-ga ee LineGene 9600 Plus ee Waqtiga-dhabta ah Wareegga Kulaylka Tirada-Waqtiga-dhabta ah ee MA-6000 Nidaamka PCR-ga ee Waqtiga-dhabta ah ee BioRad CFX96 Nidaamka PCR-ga ee waqtiga-dhabta ah ee BioRad CFX Opus 96 |
Socodka Shaqada
Waxyaabaha lagu taliyay ee soo saarista: Qalabka Guud ee DNA/RNA ee Macro & Micro-Test (HWTS-3019) (kaas oo loo isticmaali karo Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-EQ011)) oo ay samaysay Jiangsu Macro & Micro-Test Med-Tech Co., Ltd. Soo saarista waa in la soo saaraa iyadoo la raacayo tilmaamaha. Mugga muunadda soo saarista waa 200μL, mugga soo saarista ee lagu taliyayna waa 100μL.
Waxyaabaha lagu taliyay ee soo saarista: Qalabka Nadiifinta DNA-da ee Wizard® Genomic (Lambarka Catalog: A1120) ee Promega, Soo saarista Aashitada Nucleic ama Soo-saarista Nadiifinta (YDP348) ee Tiangen Biotech(Beijing) Co., Ltd. waa in la soo saaraa iyadoo la raacayo tilmaamaha soo saarista, mugga soo saarista ee lagu taliyayna waa 200 μL mugga soo saarista ee lagu taliyayna waa 160 μL.
