Human Cytomegalovirus (HCMV) Nucleic Acid
Magaca alaabta
HWTS-UR008A-Cytomegalovirus aadanaha (HCMV) xirmada ogaanshaha acid nucleic (Fluorescence PCR)
Epidemiology
Bani'aadamka cytomegalovirus (HCMV) waa xubin leh genome-ka ugu weyn qoyska fayraska herpes wuxuuna kaydin karaa in ka badan 200 oo borotiin ah.HCMV si yar ayay u xaddidan tahay inta ay martida u tahay ee bini'aadamka, walina ma jiro nooc xayawaan ah oo caabuqa ah.HCMV waxay leedahay wareeg taran tartiib ah oo dheer si ay u samayso jidhka ka mid noqoshada intranuclear, oo kicisa wax soo saarka perinuclear iyo cytoplasmic jirka ka mid ah iyo bararka unugyada (unugyada waaweyn), markaa magaca.Sida laga soo xigtay heterogeneity ee genome iyo phenotype, HCMV waxaa loo qaybin karaa noocyo kala duwan oo nuucyo ah, kuwaas oo ay ka mid yihiin kala duwanaansho antigenic gaar ah, kuwaas oo, si kastaba ha ahaatee, aan lahayn muhiimad caafimaad.
Infekshanka HCMV waa infekshin nidaamsan, kaas oo kiliinikada ku lug leh xubno badan, leh calaamado adag oo kala duwan, badanaa waa aamusan, waxayna keeni kartaa bukaano yar inay yeeshaan nabarro xubno badan oo ay ka mid yihiin retinitis, cagaarshow, oof-wareen, encephalitis, colitis, monocytosis, iyo trombocytopenic. purpuraCaabuqa HCMV aad buu u badan yahay wuxuuna u muuqdaa inuu ku faafo adduunka oo dhan.Aad ayey ugu badan tahay dadka, iyadoo heerarka dhacdooyinka ay yihiin 45-50% iyo in ka badan 90% dalalka horumaray iyo kuwa soo koraya, siday u kala horreeyaan.HCMV waxay ku jiifsan kartaa jidhka muddo dheer.Marka difaaca jirku daciifo, fayrasku waxa la hawlgelinayaa si uu cudurro u keeno, gaar ahaan caabuqyada soo noqnoqda ee bukaannada leukemia-ga ah iyo bukaannada la tallaalo, waxana uu sababi karaa necrosis xubin la tallaalo oo khatar gelinaya nolosha bukaannada xaaladaha daran.Marka lagu daro dhalmo-dhalasho, dhicin iyo dhalmo dhicis ah iyada oo loo marayo caabuqa intrauterine, cytomegalovirus sidoo kale waxay keeni kartaa cillado lagu dhasho, sidaas darteed caabuqa HCMV wuxuu awood u leeyahay inuu saameeyo daryeelka dhalmada ka hor iyo dhalmada ka dib iyo tayada dadweynaha.
Kanaalka
FAM | HCMV DNA |
VIC(HEX) | Xakamaynta Gudaha |
Qiyaasaha Farsamada
Kaydinta | Dareeraha: ≤-18℃ Madow |
Nolosha shelf | 12 bilood |
Nooca Tuunada | Muunada Serum, Muuska Plasma |
Ct | ≤38 |
CV | ≤5.0% |
LoD | 50 Nuqul / falcelin |
Gaar ahaan | Ma jiro falcelin iskutallaab ah oo leh fayraska cagaarshow B, fayraska cagaarshow C, fayraska papilloma fayraska, fayraska herpes simplex nooca 1, fayraska herpes simplex nooca 2, muunadaha serum caadiga ah ee aadanaha, iwm. |
Qalabka lagu dabaqi karo: | Waxay la jaan qaadi kartaa qalabka PCR-ka ee caadiga ah ee suuqa. Nidaamyada noolaha ee la dabaqay 7500 Nidaamyada PCR-waqtiga-dhabta ah Nidaamyada noolaha ee la dabaqay 7500 Nidaamyada PCR-waqtiga-dhabta ah ee degdegga ah QuantStudio®5 Nidaamyada PCR-waqtiga-dhabta ah SLAN-96P Nidaamyada PCR-waqtiga-dhabta ah LightCycler®480 Nidaamka PCR-waqtiga-dhabta ah LineGene 9600 Plus-Waqtiga dhabta ah ee PCR Nidaamka ogaanshaha MA-6000-waqtiga-dhabta ah ee cabbirka kulaylka wareegga BioRad CFX96 Nidaamka PCR-waqtiga-dhabta ah BioRad CFX Opus 96 Nidaamka PCR-waqtiga-dhabta ah |
Socodka Shaqada
HWTS-3017-50, HWTS-3017-32, HWTS-3017-48, HWTS-3017-96) Jiangsu Macro & Micro-Test Med-Tech Co., Ltd.. Soo saarista waa in lagu soo saaraa si waafaqsan tilmaamaha.Mugga muunadda soo saariddu waa 200μL, mugga elution ee lagu taliyeyna waa 80μL.
Soo saarista lagu taliyay: QIAamp DNA Mini Kit (51304), Soosaarka Asiidhka Nucleic ama Reagent Nadiifinta (YDP315) ee Tiangen Biotech(Beijing) Co., Ltd.waa in la soo saaraa iyadoo la raacayo tilmaamaha soo saarista, iyo mugga soo saarista lagu taliyey waa 200 μL iyo mugga sare ee lagu taliyey waa 100 μL.