Qalabka ogaanshaha Asiidhka Nucleic-ka ee Human Leukocyte Antigen B27

Bani'aadamka Leukocyte Antigen B27 Qalabka Lagu Garto Ashitada Nukliyeerka Sawirka

Sharaxaad Gaaban:

Qalabkan waxaa loo isticmaalaa in lagu ogaado tayada DNA-da ee unugyada unugyada leukocyte antigen-ka HLA-B*2702, HLA-B*2704 iyo HLA-B*2705.

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Magaca alaabta

HWTS-GE011 Human Leukocyte Antigen B27 Qalabka Lagu Ogaado Asiidhka Nucleic (Fluorescence PCR)

Epidemiology

Ankylosing spondylitis (AS) waa cudur barar barar ah oo daba-dheeraaday kaasoo inta badan ku dhaca laf dhabarta waxaana laga yaabaa inuu ku lug yeesho kala-goysyada sacroiliac iyo kala-goysyada ku xeeran ilaa heerar kala duwan.Waxa la daaha ka qaaday in AS ay muujiso isu-ururinta qoyska oo cad oo ay si dhow ula xidhiidhaan antigen-ka leukocyte HLA-B27.Bini'aadamka, in ka badan 70 nooc oo HLA-B27 ah ayaa la helay oo la aqoonsaday, waxaana ka mid ah, HLA-B*2702, HLA-B*2704 iyo HLA-B*2705 waa noocyada ugu badan ee la xidhiidha cudurka.Shiinaha, Singapore, Japan iyo degmada Taiwan ee Shiinaha, nooca-hoosaadka ugu caansan ee HLA-B27 waa HLA-B*2704, oo xisaabiya qiyaastii 54%, oo ay ku xigto HLA-B*2705, taas oo xisaabisa qiyaastii 41%.Qalabkani wuxuu ku ogaan karaa DNA-da nooc-hoosaadyada HLA-B*2702, HLA-B*2704 iyo HLA-B*2705, laakiin midba midka kale kama sooco.

Kanaalka

FAM	HLA-B27
ROX	Xakamaynta Gudaha

Qiyaasaha Farsamada

Kaydinta	Dareeraha: ≤-18℃
Nolosha shelf	Dareeraha: 18 bilood
Nooca Tuunada	shaybaar dhiig oo dhan
Ct	≤40
CV	≤5.0%
LoD	1ng/μL
Gaar ahaan	Natiijooyinka baaritaanka ee xirmadani ma saameyn doonto hemoglobin (<800g/L), bilirubin (<700μmol/L), iyo dufannada dhiigga/ triglycerides (<7mmol/L) ee dhiigga.
Qalabka lagu dabaqi karo	Nidaamyada noolaha ee la dabaqay 7500 Nidaamyada PCR-waqtiga-dhabta ah Hababka noolaha ee la dabaqay StepOne Nidaamyada PCR-waqtiga-dhabta ah LightCycler^®480 Nidaamka PCR-waqtiga dhabta ah Agilent-Stratagene Mx3000P Nidaamka Q-PCR