Qaamo-qashiirta Virus Nucleic Acid
Magaca alaabta
HWTS-RT029-Qashin-qashiirta Qalabka Lagu Ogaado Ashitada Nukliyeerka (Fluorescence PCR)
Epidemiology
Fayraska qaamo-qashiirta waa fayras keli ah oo serotype ah, laakiin hidda-wadaha borotiinka SH ayaa aad ugu kala duwan fayraska qaamo-qashiirta. Fayraska qaamo-qashiirta waxa loo qaybiyaa 12 genotypes oo ku salaysan kala duwanaanshaha hidda-sidaha borotiinka SH, kuwaas oo kala ah noocyada A, B, C, D, F, G, H, I, J, K, L, iyo N. Qaybinta fayraska qaamo-qashiirta genotypes waxa ay leedahay sifooyin gobol oo cad. Noocyada ku baahsan Yurub waxay u badan yihiin genotypes A, C, D, G, iyo H; Noocyada ugu badan ee Ameerika waa genotypes C, D, G, H, J, iyo K; Noocyada ugu badan ee Aasiya waa genotypes B, F, I, iyo L; Cadaadiska ugu weyn ee Shiinaha waa genotype F; Noocyada baahsan ee Japan iyo South Korea waa genotypes B iyo siday u kala horreeyaan. Ma cadda in qoritaanka fayraska ku salaysan SH uu macno u leeyahay cilmi-baarista tallaalka. Waqtigan xaadirka ah, noocyada tallaalka nool ee la dhimay ee adduunka oo dhan laga isticmaalo ayaa u badan genotype A, iyo unugyada difaaca jirka ee ay soo saaraan antigens-ka fayrasyada ee genotypes kala duwan ayaa iska ilaaliya.
Qiyaasaha Farsamada
| Kaydinta | -18℃ |
| Nolosha shelf | 9 bilood |
| Nooca Tuunada | Dhuunta dhuunta |
| Ct | ≤38 |
| CV | ≤5.0% |
| LoD | 1000 koobi/ml |
| Qalabka lagu dabaqi karo | Lagu dabaqi karo nooca I ogaanshaha reagent: Nidaamyada noolaha ee la dabaqay 7500 Nidaamyada PCR-waqtiga-dhabta ah, QuantStudio®5 Nidaamyada PCR-waqtiga-dhabta ah, SLAN-96P Nidaamyada PCR-Waqtiga-dhabta ah (Hongshi Medical Technology Co., Ltd.), Nidaamyada ogaanshaha PCR ee LineGene 9600 Plus-waqtiga-dhabta ah (FQD-96A, farsamada Hangzhou Bioer), MA-6000-Waqtiga-dhabta ah ee Heerkulbeegga Heerarka Heerarka (Suzhou Molarray Co., Ltd.), BioRad CFX96 Nidaamka PCR-waqtiga-dhabta ah, BioRad CFX Opus 96 Nidaamka PCR-waqtiga-dhabta ah. Lagu dabaqi karo nooca II reagent ogaanshaha: EudemonTMAIO800 (HWTS-EQ007) ee Jiangsu Macro & Micro-Test Med-Tech Co., Ltd. |
Socodka Shaqada
Macro & Micro-Test Viral DNA/RNA Kit (HWTS-3017) (kaaso loo isticmaali karo Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006C, HWTS-3006B)), iyo Macro & Micro-Test Viral DNA/RNA Kit (HWTS-3017-8)TM AIO800 (HWTS-EQ007)) ee Jiangsu Macro & Micro-Test Med-Tech Co., Ltd.
Mugga muunadda la soo saaray waa 200μL iyo mugga sareynta lagu taliyey waa 150μL.
